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[GreenB] _____ Platinum HM Polymerase

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  • 작성자 :최고관리자
  • 작성일 :작성일19.03.22
  • 조회수 :1,186
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Platinum HM Polymerase 

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Taq Platinum DNA Polymerase is ideal for automatic “hot-start” amplification of  DNA fragments with improved specificity over Taq DNA polymerase. It is derived  from recombinant Taq DNA polymerase by binding of a thermolabile inhibitor  containing chemical compound to Taq DNA polymerase.

Taq Platinum DNA  Polymerase has a 3’→5’ Exonuclease Activity as well as 5’→3’ Exonuclease  Activity. During the initial denaturation step of PCR, the inhibitor is denatured  and active Taq DNA polymerase is released into the reaction. With Taq Platinum  DNA Polymerase, improved specificity and yields of PCR compared to Taq DNA  polymerase without “hot-start” could be reached.

HotMaster Taq DNA Polymerase is a superior alternative for performing PCR experiments generally known as “hot start” PCR. HotMaster blocks the substrate binding site of DNA polymerases in a temperature-dependent manner. Inactive polymerase-inhibitor complexes are formed at temperatures < 40°C, where the affinity of HotMaster for Taq polymerase is higher than the binding affinity of the template DNA . Between 40°C and 55°C the HotMaster competes with the template DNA for binding to the Taq polymerase, thereby shifting the binding equilibrium towards complex formation with only target-specific primed template DNA . At temperatures above 55°C the HotMaster inhibitor is displaced from complexes with the Taq polymerase by target-specific primed template DNA. A unique performance feature of the HotMaster inhibitor is that it can go through multiple temperature cycles of binding-equilibrium competition-dissociation during PCR without irreversible heat inactivation. Where other Taq polymerase
formulations for “hot start” PCR block the activity of Taq polymerase only prior to the first high temperature step, the TIANGEN HotMaster provides sustained temperature control throughout PCR.​