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[GreenB] _____ HotMaster easy Taq DNA Polymerase

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  • 작성자 :최고관리자
  • 작성일 :작성일19.05.20
  • 조회수 :943
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HotMaster easy Taq DNA Polymerase



HotMaster Taq DNA Polymerase is a superior alternative for performing PCR experiments generally known as “hot start” PCR. HotMaster blocks the substrate binding site of DNA polymerases in a temperature-dependent manner. Inactive polymerase-inhibitor complexes are formed at temperatures < 40°C, where the affinity of HotMaster for Taq polymerase is higher than the binding affinity of the template DNA . Between 40°C and 55°C the HotMaster competes with the template DNA for binding to the Taq polymerase, thereby shifting the binding equilibrium towards complex formation with only target-specific primed template DNA . Where other Taq polymerase formulations for “hot start” PCR block the activity of Taq polymerase only prior to the first high temperature step, the TIANGEN HotMaster provides sustained temperature control throughout PCR.

EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. EasyTaq®DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.