제품명 |
GelStain |
브랜드 |
TransGen |
분류 |
전기영동 |
제품번호 |
GS101-01 |
규격 |
500 ?l |
제품 상세정보
GelStain is a sensitive , stable and relatively safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA , ssDNA or RNA in agarose gels or polyacrylamide gels.
The sensitivity of GelStain is much higher than EB , and destaining is not required.
GelStain and EB have virtually the same spectral properties.
Highlights
? Nontoxic: Unique lipophilic and macromolecular properties of GelStain make it incapable of penetrating cell membranes.
Ames test also shows the mutagenicity of GelStain is far less than that of EB.
? Highly sensitive: Suitable for staining fragments of different sizes in electrophoresis gel.
? Highly stable: Suitable for using microwave or other heating methods to prepare agarose gel.
? High signal-to-noise ratio: Strong fluorescence signal of the sample with low background signal.
? Simple operation: Similar to ethidium bromide , the dye does not degrade during the process of preparing gel or electrophoresis.
It only takes 30 minutes for staining after electrophoresis , and the fragment can be visualized by a UV-transilluminator directly without detaining or washing.
? A broad range of applications: Applicable for precast protocol (add dye during gel preparation) and post-stain protocol (submerge the gel in the staining solution); suitable for agarose gels and polyacrylamide gels electrophoresis; suitable for staining dsDNA , ssDNA or RNA.
? Changing the existing imaging system is not required: Suitable for both standard EB filter and SYBR filter; same with the standard UV-transilluminator used for EB staining observation; optimal excitation in the UV region around 300 nm.
Concentration
10000×
Storage
at room temperature protected from light for two years.
Shipping
at room temperature
제품 참고자료 : http://www.transgenbiotech.com/data/upload/pdf/GS101_2024-05-30.pdf