제품소개
TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG
| 제품명 | TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG |
|---|---|
| 브랜드 | TransGen |
| 분류 | qPCR |
| 제품번호 | AQ322-01 |
| 규격 | 100 rxns��20 ��l system |
제품 상세정보
TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG is designed for one-step qRT-PCR with high sensitivity , high synthesis efficiency and high amplification efficiency.
This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers , and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube.
After special optimization , this kit enables consistently amplifying multiple targets in a single reaction ensuring multiplex one-step RT-qPCR.
dUTP/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA , which can prevent cross contamination.
Highlights
? Use TransScript® II Multiplex Probe One-Step Enzyme Mix UDG , 2×®PerfectStart® Multiplex Probe One-Step Reaction Mix to efficiently synthesize RNA into first-strand cDNA for qPCR.
It is easy to operate and reduces the chance of contamination during operation.
? Using UDG enzyme and dUTP to effectively prevent cross-contamination of PCR products with accurate data.
? High sensitivity , high specificity and the data are accurate.
Applications
? Singleplex to 4-plex RT-qPCR detection.
? High-copy and low-copy gene detection.
? RNA templates with high GC content or complex secondary structure.
? Detection of RNA virus or trace amounts of RNA.
Storage
Store at -20 ??for two years
Shipping
Dry ice (-70??
This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers , and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube.
After special optimization , this kit enables consistently amplifying multiple targets in a single reaction ensuring multiplex one-step RT-qPCR.
dUTP/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA , which can prevent cross contamination.
Highlights
? Use TransScript® II Multiplex Probe One-Step Enzyme Mix UDG , 2×®PerfectStart® Multiplex Probe One-Step Reaction Mix to efficiently synthesize RNA into first-strand cDNA for qPCR.
It is easy to operate and reduces the chance of contamination during operation.
? Using UDG enzyme and dUTP to effectively prevent cross-contamination of PCR products with accurate data.
? High sensitivity , high specificity and the data are accurate.
Applications
? Singleplex to 4-plex RT-qPCR detection.
? High-copy and low-copy gene detection.
? RNA templates with high GC content or complex secondary structure.
? Detection of RNA virus or trace amounts of RNA.
Storage
Store at -20 ??for two years
Shipping
Dry ice (-70??
제품 참고자료 : http://www.transgenbiotech.com/data/upload/pdf/AQ322_2024-05-30.pdf
