제품소개
2×®TransTaq® FastPfu Fly PCR SuperMix(-dye)
| 제품명 | 2×®TransTaq® FastPfu Fly PCR SuperMix(-dye) |
|---|---|
| 브랜드 | TransGen |
| 분류 | PCR |
| 제품번호 | AS231-01 |
| 규격 | 1 ml |
제품 상세정보
This product contains TransTaq® FastPfu Fly DNA Polymerase , dNTPs and optimized reaction buffer at a concentration of 2×®.
It has the features of high amplification efficiency and fast amplification speed (?? kb fragments can achieve 12 kb/min extreme amplification , > 5 kb fragments can achieve 6 kb/min high-speed amplification).
The SuperMix is provided at 2×® concentration and can be used at 1× concentration by adding template , primers and Nuclease-free Water for amplification.
The amplified product of 2×®TransTaq®FastPfu Fly PCR SuperMix (-dye) can be cloned directly into pEASY®-Blunt series of vectors.
The amplified product of 2×®TransTaq®FastPfu Fly PCR SuperMix (+dye) can be analyzed by electrophoresis directly , and need to be purified to remove dye when applied in cloning.
? Reduce PCR operation time.
?Avoid contamination caused by multi-step operation.
?TransTaq® FastPfu Fly PCR SuperMix offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
?Amplification of genomic DNA fragment up to 15 kb.
?Amplification of plasmid DNA fragment up to 20 kb.
Highlights
Fast , ultra-high fidelity , high specificity , good stability.
Applications
? Ultra high fidelity PCR
? Site-directed mutagenesis
? Blunt end cloning
? Complex templates
? GC/AT-rich templates
? Long fragment amplification
Storage
at -20 ??for two years
Shipping
Dry ice (-70??
It has the features of high amplification efficiency and fast amplification speed (?? kb fragments can achieve 12 kb/min extreme amplification , > 5 kb fragments can achieve 6 kb/min high-speed amplification).
The SuperMix is provided at 2×® concentration and can be used at 1× concentration by adding template , primers and Nuclease-free Water for amplification.
The amplified product of 2×®TransTaq®FastPfu Fly PCR SuperMix (-dye) can be cloned directly into pEASY®-Blunt series of vectors.
The amplified product of 2×®TransTaq®FastPfu Fly PCR SuperMix (+dye) can be analyzed by electrophoresis directly , and need to be purified to remove dye when applied in cloning.
? Reduce PCR operation time.
?Avoid contamination caused by multi-step operation.
?TransTaq® FastPfu Fly PCR SuperMix offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
?Amplification of genomic DNA fragment up to 15 kb.
?Amplification of plasmid DNA fragment up to 20 kb.
Highlights
Fast , ultra-high fidelity , high specificity , good stability.
Applications
? Ultra high fidelity PCR
? Site-directed mutagenesis
? Blunt end cloning
? Complex templates
? GC/AT-rich templates
? Long fragment amplification
Storage
at -20 ??for two years
Shipping
Dry ice (-70??
제품 참고자료 : http://www.transgenbiotech.com/data/upload/pdf/AS231_2024-05-30.pdf
