제품소개
| 제품명 | pEASY-Basic Seamless Cloning and Assembly Kit |
|---|---|
| 브랜드 | Transgen |
| 분류 | Cloning |
| 제품번호 | CU201-02 |
| 규격 | 20rxns |
This kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-5 fragments.
• Fast: 5~15 minutes.
• Simple: No restriction enzyme digestions.
• High efficiency: > 95% cloning efficiency.
• Seamless: No extra sequences introduced.
Storage
at -20°C for one year.
Shipping
Dry ice (-70℃)
| Component | CU201-02 | CU201-03 | CU201-13 |
| 2×Basic Assembly Mix | 100 μl | 3×100 μl | - |
| 2×Basic Assembly Mix (+Dye) | - | - | 3×100 μl |
| Linearized pUC19 Control Vector (10 ng/μl) | 6 μl | 18 μl | 18 µl |
| Control Insert (1 kb,20 ng/μl) | 6 μl | 18 μl | 18 µl |
